Journal: FEMS Microbiology Ecology
Article Title: Challenging the paradigm: non-canonical exoprotease cheating in clinical Pseudomonas aeruginosa isolates
doi: 10.1093/femsec/fiaf106
Figure Lengend Snippet: Competitions of AUS 531 and the derived non-exoprotease producers 24.7 and 34.7. The initial percentage of the non-exoprotease producers at the beginning of the experiments and after 24 h are shown, for comparison of the competence between PAO1, and its isogenic lasR mutant is shown. Competitions were performed in triplicate, and the average of the initial and 24 h percentages of non-exoprotease producers ± SEM are shown. Asterisk indicates significant differences ( P ˂ 0.05 in a two-tailed t -test).
Article Snippet: Under this model, continuous growth with protein as sole carbon source selects for lasR mutants that cease exoprotease production, exploiting cooperators and often triggering population collapses—a phenomenon framed as a microbial “tragedy of the commons” (Sandoz et al. , Dandekar et al. , Loarca et al. ).
Techniques: Derivative Assay, Comparison, Mutagenesis, Two Tailed Test